Table 3. pEC₅₀ and I_max values of ACh, acetamiprid, and imidacloprid for wild-type, T77R, and T77R+E79V Dα2β2 nAChRs.

Compounds	Dα2β2 WT		Dα2β2 T77R		Dα2β2 T77R+E79V
	pEC50	Imax
Ach	4.60±0.04	1.08±0.03	4.64±0.07	1.03±0.01	4.47±0.03	1.04±0.02
Acetamiprid	5.26±0.09	0.49±0.03	5.42±0.06	0.99±0.04*	5.38±0.04	0.97±0.03*
Imidacloprid	5.61±0.06	0.77±0.03	6.35±0.06*	0.79±0.03	6.18±0.02*	0.96±0.02*

Values shown are the results of a fit of the concentration–response data (mean±S.E.M., n=4). Statistical test (one-way ANOVA, Dunnett’s multiple-comparison test) is for significant differences from the wild-type data. * p<0.05. The peak amplitude of the current recorded in the response to each challenge was normalized to the maximum amplitude of the response to ACh. Data from the Dα2β2, T77R, and T77R+E79V mutants were normalized to the response to 1 mM ACh.