Table 1.
Major features of plasmids analyzed
| Category | Plasmid
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| pP10159-1 | pNDM-HN380 | pP10159-2 | pP378-IMP | pP10159-3 | pHS062105-3 | pECN49-KPC | pP10159-4 | pNDM-CIT | pP10159-5 | pR55 | |
| Incompatibility group | IncX3 | IncN1 | Unknown | IncHI4 | Type 2 IncC | ||||||
| Total length (bp) | 54,034 | 54,035 | 51,104 | 51,207 | 42,763 | 42,848 | 41,317 | 228,709 | 288,920 | 152,132 | 170,810 |
| Total number of ORFs | 62 | 62 | 62 | 64 | 53 | 53 | 52 | 270 | 314 | 190 | 203 |
| Mean G + C content, % | 49.0 | 49.0 | 50.4 | 50.5 | 49.8 | 49.7 | 49.1 | 47.0 | 47.7 | 51.1 | 53.0 |
| Length of the backbone (bp) | 34,731 | 34,732 | 37,474 | 37,345 | 29,082 | 29,043 | 29,043 | 190,385 | 208,301 | 89,438 | 129,510 |
| Accessory modules | The blaNDM-1 region# and ISKox3 | In823b#, ΔTn6292# and the IS1 remnant | ΔTn6296# | The MDR region#, the blaCTX-M-3 region#, ISLad2, ISKpn26-IS1R, ISCfr4-ΔISCfr9, IS903B and ISCfr6:ISEc21 | The MDR region#, ΦCP4-6/57#, ISKpn26, ISKpn34, ΔISCfr9 and ISSen3 | The MDR region#, ΔTn6292-3′ and two separate copies of ISEc52 | The floR-ISCR2 region# and Tn6187# | ||||
Notes: pP10159-1, pP10159-2, pP10159-3, pP10159-4 and pP10159-5 from C. freundii P10159 were sequenced in this work, while the other plasmids were derived from GenBank and used as references. Sequence comparison of each of these five sequenced plasmids with their reference plasmid(s) was interpreted in the text.
#
These accessory modules contained resistance genes as listed in Table 2.
Abbreviation: MDR, multi-drug resistant.