Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) |
WT (w1118) | NA | NA | |
| Genetic reagent (D. melanogaster) |
Sca-Gal4 | PMID: 2125959 | NA | |
| Genetic reagent (D. melanogaster) |
mitoDendra2 | Dr. Thomas Rival (University of Marseille) |
NA | |
| Genetic reagent (D. melanogaster) |
aurA ST | PMID: 23685146 | NA | |
| Genetic reagent (D. melanogaster) |
aurA 3A | this paper | NA | aurA3A indel |
| Genetic reagent (D. melanogaster) |
aurA 2G | this paper | NA | aurA2G indel |
| Genetic reagent (D. melanogaster) |
aurA dsRNA | Vienna Drosophila RNAi Center |
VDRC: 108446 | |
| Genetic reagent (D. melanogaster) |
aurA exel | Bloomington Drosophila Stock Center |
BDSC: 8376 | |
| Genetic reagent (D. melanogaster) |
aurA exel | Bloomington Drosophila Stock Center |
BDSC: 8377 | |
| Genetic reagent (D. melanogaster) |
Drp1 | Bloomington Drosophila Stock Center |
BDSC: 51647 | |
| Cell line (H. sapiens) | MCF7 | ATCC | HTB-22 | Maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L-Glutamine |
| Cell line (H. sapiens) | HEK293 | ATCC | CRL-1573 | Maintained in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L-Glutamine |
| Cell line (H. sapiens) | Hs578T | ATCC | HTB-126 | Maintained in the Legembre Lab in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L- Glutamine |
| Cell line (H. sapiens) | MDA-MB-231 | ATCC | HTB-26 | Maintained in the Legembre Lab in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L- Glutamine |
| Cell line (H. sapiens) | MDA-MB-468 | ATCC | HTB-132 | Maintained in the Legembre Lab in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L- Glutamine |
| Cell line (H. sapiens) | T47D | ATCC | HTB-133 | Maintained in the Legembre Lab in DMEM supplemented with 10% FBS, 1% penicillin/streptomycin and 1% L- Glutamine |
| Antibody | AURKA | Merck Millipore | PC742 | 1:1000 |
| Antibody | AURKA | home made | clone 5C3 (ref. 48 of this paper) |
1:20 |
| Antibody | Drp1 | BD Pharmingen | 611112 | 1:2000 |
| Antibody | Drp1 pS616 (DA91) | Cell Signaling | 4494 | 1:1000 |
| Antibody | Drp1 pS637 | Cell Signaling | 4867 | 1:1000 |
| Antibody | Fis1 (TTC1) clone EPR8412 | abcam | ab156865 | 1:1000 |
| Antibody | GFP | Roche/Sigma Aldrich | 11814460001 | 1:1000 |
| Antibody | Histone H3 pS10 | Millipore | 06–570 | 1:10,000 |
| Antibody | His tag | Covalab | mab90001-P | 1:3000 in 3% BSA |
| Antibody | Mff | abcam | ab81127 | 1:500 |
| Antibody | MitoFusin 1 | abcam | ab57602 | 1:1000 |
| Antibody | MitoFusin 2 | abcam | ab56889 | 1:500 |
| Antibody | Opa1 | abcam | ab157457 | 1:1000 |
| Antibody | OXPHOS Rodent WB Antibody Cocktail |
Mitosciences | ab110413 | 1:1000 |
| Antibody | PMPCB | Proteintech | 16064–1-AP | 1:1000 |
| Antibody | RalA S194 | Millipore | 07–2119 | 1:1000 |
| Antibody | Tom22 | Abcam | ab10436 | 1:500 |
| Antibody | Tom70 | Abcam | ab106193 | 1:5000 |
| Antibody | Tubulin alpha clone YL1/2 | Millipore | MAB1864 | 1:5000 |
| Antibody | VDAC1 | Abcam | ab15895 | 1:1000 |
| Chemical compound, drug | MLN8237/Alisertib | Selleck chemicals | S1133 | |
| Chemical compound, drug | TMRM | Thermo Fischer scientific | T668 | |
| Sequence-based reagent | Allstar Control siRNA | Qiagen | SI03650318 | |
| Sequence-based reagent | AURKA siRNA | Eurogentec | NA | sequence 5'-AUGCCCUG UCUUACUGUCA-3' |
| Sequence-based reagent | RALA siRNA | Qiagen | SI03650318 | |
| Sequence-based reagent | PMPCB siRNA | Dharmacon | L-004747- 00–0005 | |
| Recombinant DNA reagent | AURKA shRNA | Sigma Aldrich | SHCLNG-NM_003600 | |
| Recombinant DNA reagent | Control shRNA | Sigma Aldrich | SHC002 |