Figure 8.

Treatment of rat hippocampal primary cultures with palbociclib did not elicit cytotoxicity.

Notes: Primary hippocampal culture treated with vehicle solution or 2 µM palbociclib for 24 hours. Intact neuronal networks and normal glial cell morphology was observed in cultures treated with palbociclib, suggesting no significant toxicity. Cell nuclei were stained with DAPI (blue); neurons were stained with B3 tubulin antibody (green); glial cells were stained with the GFAP antibody (red/orange).

Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; B3 tubulin, tubulin, beta III isoform; GFAP, glial fibrillary acidic protein.