Fig. 6.

Analysis of GBM12 and GBM12 TMZ-resistant cell migration capacity and the effect of Fn14 depletion on GBM12 TMZ-resistant cell migration. (A) Parental GBM12 cells and matched GBM12 TMZ-resistant cells (GBM12 TMZ) transfected with either nontargeting (NT) siRNA or Fn14 siRNA were harvested and lysed. Fn14 and GAPDH levels were examined by western blot analysis. (B) GBM12 TMZ cells were transfected with either NT siRNA or Fn14 siRNA and 48 h later these cells and the parental GBM12 cells were cultured in serum-free medium for 16 h and then plated on transwell migration chambers. Medium containing 10% serum was placed in the lower wells to act as a chemoattractant. After 4 h of incubation, the number of cells that migrated through the membrane was determined. Values shown are the mean ± SD of triplicate chambers. Significance was measured by Student’s t-test. *P < 0.05, **P < 0.01. The difference between the GBM12 and GBM12 TMZ plus Fn14 siRNA values is not significant.