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Thermal unfolding based on intrinsic fluorescence data. Squares—T4 lysozyme in solution. Triangles—T4 lysozyme adsorbed onto adjuvant. The apparent Tms are approximately 64 ± 0.5°C and 43°C for the solution state and adsorbed protein, respectively. For comparison, the apparent Tms of these formulations according to DSC data (thermograms not shown) were 65 ± 0.5°C (solution state) and 45 ± 0.10°C (adsorbed).
