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. Author manuscript; available in PMC: 2018 Sep 17.
Published in final edited form as: Cell Rep. 2018 Feb 6;22(6):1545–1559. doi: 10.1016/j.celrep.2018.01.026

Figure 3. Yy1 Deficiency Results in Reduced HSC Long-Term Repopulating Activity in Competitive Serial Transplantations.

Figure 3.

Mx1-cre or Yy1f/f Mx1-cre bone marrow was mixed with CD45.1+ bone marrow competitor cells in a 1:1 or 9:1 ratio and transplanted into lethally irradiated CD45.1+ recipients. Four weeks after transplantation, recipient mice received 5 doses of pI-pC to delete the endogenous YY1.

(A) Lineage evaluations of donor-derived contribution in primary total peripheral blood cell, myeloid, T cells, and B cells at 4, 8, 12, and 16 weeks post BMTs.

(B) Donor-derived contribution in primary total bone marrow, spleen, and peripheral blood cells.

(C) Donor-derived contribution in primary bone marrow LSK, LT-HSC, ST-HSC, MPP, and MP.

(D) Donor-derived contribution in secondary total peripheral blood, myeloid, T and B cell.

(E) Donor-derived contribution in secondary total bone marrow, spleen, peripheral blood cells, bone marrow LSK, LT-HSC, ST-HSC, MPP, and MP. N represents the number of mice; graphs show means ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.