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. Author manuscript; available in PMC: 2018 Sep 17.
Published in final edited form as: Cell Rep. 2018 Feb 6;22(6):1545–1559. doi: 10.1016/j.celrep.2018.01.026

Figure 4. YY1 Regulation of HSC Long-Term Self-Renewal Is Independent on its PcG Function.

Figure 4.

(A) Experimental strategy: Yy1f/f Mx1-Cre bone marrow cells transduced with MigR1, MigR1-Flag-YY1, or MigR1-Flag-ΔREPO, or Mx1-Cre bone marrow cells transduced with MigR1, was injected into lethally irradiated CD45.1+ congenic mice. Five doses of pI-pC injections were given at 4 weeks post BMTs. Bone marrows from reconstituted mice were harvested for analyses or for secondary BMTs at 20 weeks post transplantation.

(B) (i and ii) Prior to BMT, flow analysis of % GFP+ and MFI of transduced BM cells at 48 hr post viral infection. (iii) Western blot to detect exogenous Flag-YY1, Flag-ΔREPO, and endogenous YY1 in GFP+ peripheral lymphocytes before and after pI-pC injections. (iv) Quantification of exogenous Flag-YY1, Flag-REPO, and endogenous YY1 protein expressions.

(C) Primary BMT evaluations of donor-derived contribution and % GFP+ in donor-derived peripheral blood (i) and total bone marrow (ii).

(D) Primary BMT evaluation of % GFP+ donor-derived LSK, LT-HSC, ST-HSC, MPP, and MP.

(E) Secondary BMT evaluation of donor-derived contribution and % GFP+ in donor-derived peripheral blood (i) and total bone marrow (ii). N represents the number of mice; graphs show means ± SEM; **p < 0.01, ***p < 0.001.