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. Author manuscript; available in PMC: 2018 Sep 17.
Published in final edited form as: Cell Rep. 2018 Feb 6;22(6):1545–1559. doi: 10.1016/j.celrep.2018.01.026

Figure 7. YY1 Promotes SCF/c-Kit Signaling.

Figure 7.

(A) Quantitative real-time PCR analysis of Yy1, Gata2, Kit, and Runx1 transcript levels in total bone marrow cells.

(B) Evaluation of c-Kit MFI in LT-HSC.

(C) Evaluation of c-Kit MFI in Lin−bone marrow cells.

(D) Phosphorylated flow analysis of AKT.

(E) YY1 occupancy at Kit. (i) “+” and “− ” indicate positive versus negative occupancy of transcription factors at Kit based on ChIP-seq (GEO: GSE22178) or ChIP-qPCR, respectively. (ii) YY1 occupancy at Kit in HPC-7 cells detected by ChIP-qPCR and normalized to input.

(F) The model assumes that YY1 directly regulates SCF/c-Kit signaling in HSCs and promotes HSC long-term self-renewal and quiescence without a requirement for the REPO domain/PcG function. By contrast, YY1 REPO domain/ PcG function is required for Ig rearrangement in early B cells. N represents the number of mice; graphs show means ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001.