Fig. 1. Increased circulating T_FH cells in PASLI/APDS patients.

Analyses of blood from healthy donors (HD n=8) and PASLI/APDS patients (n=7). a, Representative flow plots and histogram of CD45RA^—CXCR5⁺ cT_FH cells (percent of CD4⁺CD3⁺CD25^— T cells). b, Representative contour plots and histogram of PD-1⁺CXCR5⁺ T cells (percent of CD4⁺CD3⁺CD25^— T cells). Right: geometric mean fluorescent intensity (MFI) of PD-1 (gated on PD-1⁺ cT_FH cells). c, Representative contour plots of CCR6⁺ and CXCR3⁺ cT_FH cells and histogram of percent of CXCR3⁺ cT_FH (of cT_FH cells). Right: MFI of CXCR3 (gated on CXCR3⁺ cT_FH cells). Data in (a-c) are represented as mean ± SEM with each dot indicating one individual. Significance analyzed by Mann-Whitney U test. *P < 0.05; **P < 0.01; ***P < 0.001.