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. Author manuscript; available in PMC: 2019 Feb 20.
Published in final edited form as: Nat Immunol. 2018 Aug 20;19(9):986–1000. doi: 10.1038/s41590-018-0182-3

Fig. 6. Pik3cdE1020K/+ mice develop autoantibodies and infiltration of multiple organs.

Fig. 6.

a, ELISA for serum ANA-IgG on 14/16-week-old wild-type (n=8) and Pik3cdE1020K/+ (n=6) mice. b, Serum IgG autoantibody array chip is shown; a colorimetric representation of relative autoantibody reactivity is shown based on 0–3 scale. Sera from 3 wild-type, 11 Pik3cdE1020K/+ and 2 lupus-prone positive control MRL/NZM mice were analyzed c, H&E staining of perfused lung, liver, kidney, pancreas from 14-month-old wild-type and Pik3cdE1020K/+ mice at steady state. Cell infiltrations are indicated with asterisks. Right, pie charts with each segment representing one mouse; grey denotes cell infiltration into the organs (scale bar 200 μm, lung and liver; 100 μm, kidney and pancreas) (n=5 per group). Data in (a) are representative of 3 independent experiments. Data in (b) were obtained from one experiment analyzing mice from multiple litters. Data in (c) were representative of 2 independent experiments. Data in (a) are expressed as mean ± SEM with each dot indicating one mouse Significance analyzed by Mann-Whitney U test. **P < 0.01.