Table 4.

Parameters related to nitrogen metabolism in rumen fluid (mg·100 mL⁻¹)

Item	MOS1				SEM2	P value
	0%	1.2%	1.6%	2.0%
Total nitrogen
Before feeding	157.60	124.80	152.00	164.80	12.19	0.733
After 1 h	160.80	165.87	201.60	177.07	13.43	0.778
After 3 h	183.20	130.13	181.87	146.40	12.48	0.354
After 5 h	145.60	153.07	149.87	160.80	12.78	0.990
After 7 h	133.60	173.33	188.00	170.40	13.12	0.646
Ammonia nitrogen
Before feeding	71.24	81.43	71.72	68.42	3.97	0.794
After 1 h	179.97	97.87	105.00	95.44	15.02	0.203
After 3 h	131.78	89.35	91.56	89.81	8.45	0.305
After 5 h	136.26	96.96	50.86	53.75	17.97	0.385
After 7 h	72.18	70.32	74.35	90.67	5.95	0.716
Protein nitrogen
Before feeding	81.69	68.87	77.19	93.48	13.58	0.960
After 1 h	66.93	65.64	94.95	111.52	11.22	0.446
After 3 h	48.76	38.19	89.25	54.44	11.03	0.360
After 5 h	43.89	54.48	115.32	105.58	14.88	0.237
After 7 h	58.78	100.08	111.25	78.49	13.96	0.679

¹Sheep were fed 0%, 1.2%, 1.6%, and 2.0% mannan-oligosaccharides (MOS) (n = 3 per treatment). Mean results of nitrogen metabolism of rumen fluid are shown for the five time point collection at seventh day of the study for each treatment.

²SEM: standard error of the mean.