Table 5.

Parameters related to pH and volatile fatty acid in rumen fluid (mM, %)

Item	MOS1				SEM2	P value
	0%	1.2%	1.6%	2.0%
pH
Before feeding	7.17	7.10	7.05	7.37	0.08	0.653
After 1 h	6.40	6.54	6.49	6.68	0.12	0.933
After 3 h	6.46	6.22	6.81	6.60	0.13	0.443
After 5 h	6.55	5.76	6.40	6.11	0.22	0.648
After 7 h	6.94	6.80	6.94	6.83	0.16	0.992
Total volatile fatty acid
Before feeding	57.48	55.58	61.20	48.67	5.29	0.916
After 1 h	95.25	67.23	90.03	69.44	9.50	0.730
After 3 h	93.69	66.28	75.26	65.94	8.36	0.747
After 5 h	69.48	55.10	110.02	87.33	14.69	0.660
After 7 h	54.87	70.45	77.61	65.07	8.74	0.907
Acetate:TVFA
Before feeding	69.83	68.84	69.51	70.94	0.66	0.810
After 1 h	67.02	67.47	69.38	67.83	0.82	0.822
After 3 h	67.56	68.61	67.74	66.96	0.74	0.924
After 5 h	66.94	70.86	68.55	66.08	1.50	0.742
After 7 h	68.70	64.50	69.83	69.82	1.28	0.396
Propionate:TVFA
Before feeding	13.34	13.23	9.55	10.61	0.82	0.242
After 1 h	21.08	17.30	16.85	18.29	1.14	0.703
After 3 h	18.59	17.01	16.95	20.54	0.94	0.601
After 5 h	18.06	14.81	15.70	22.34	1.58	0.395
After 7 h	13.48	20.72	14.13	13.86	1.52	0.240
Butyrate:TVFA
Before feeding	12.21a	10.43b	12.89a	12.42a	0.35	0.001
After 1 h	9.58	10.82	10.31	9.91	0.48	0.878
After 3 h	10.02	10.87	11.54	9.45	0.35	0.156
After 5 h	11.14	11.61	12.04	8.56	0.54	0.073
After 7 h	12.82	10.72	10.37	12.13	0.41	0.113
Acetate:Propionate
Before feeding	5.24	5.47	7.39	6.69	0.42	0.202
After 1 h	3.22	4.07	4.14	4.02	0.28	0.760
After 3 h	3.67	4.06	4.12	3.38	0.22	0.716
After 5 h	3.87	5.15	4.39	3.14	0.49	0.578
After 7 h	5.11	3.28	5.41	5.40	0.53	0.420

¹Sheep were fed 0%, 1.2%, 1.6%, and 2.0% mannan-oligosaccharides (MOS) (n = 3 per treatment). Mean results of pH and volatile fatty acid of rumen fluid are shown for the five time point collection at seventh day of the study for each treatment.

²SEM = standard error of the mean.

^a,bMeans within rows with different superscript letters differ (P < 0.05).