. 2018 Apr 13;96(4):1305–1316. doi: 10.1093/jas/sky037

Table 3.

Effect of VA on selenoproteins, inflammatory cytokines, Nrf2 and NF-κB pathway mRNA expression in DETA-induced BMEC

Index	Treatment										SEM	P-value
Index	Negative control	DETA	0.05VA + DETA	0.1VA + DETA	0.2VA + DETA	0.5VA + DETA	1VA + DETA	2VA + DETA	3VA + DETA	4VA + DETA	SEM	Treatment	Linear	Quadratic
GPx1	1.382^ab	0.510^c	1.136^bc	1.242^bc	1.600^ab	1.720^ab	2.110^a	1.738^ab	1.114^bc	1.001^bc	0.171	0.0097	0.4877	0.0110
GPx4	0.950^de	0.610^f	1.064^cde	1.200^bcd	1.380^abc	1.461^ab	1.647^a	1.372^abc	0.893^def	0.789^ef	0.074	<0.0001	0.2978	0.0068
TrxR1	1.001^c	0.427^d	0.865^cd	1.512^b	1.701^b	1.929^b	2.469^a	2.010^ab	1.865^b	1.815^b	0.143	<0.0001	0.0100	0.0003
IL-1β	0.969^d	4.404^a	4.242^a	3.004^b	2.724^bc	3.037^b	1.884^c	2.635^bc	2.687^bc	2.006^c	0.283	<0.0001	0.0016	0.0018
IL-6	1.011^d	2.013^a	1.509^bc	1.357^bcd	1.214^cd	1.240^cd	1.133^cd	1.195^cd	1.701^ab	1.378^bcd	0.131	0.0004	0.7827	0.2487
TNF-α	1.003^abc	1.207^a	1.015^abc	0.833^bc	0.831^bc	0.858^abc	0.706^c	0.872^abc	1.081^ab	0.869^abc	0.101	0.1045	0.7745	0.6732
iNOS	0.920^ab	1.104^a	0.641^bc	0.482^cd	0.470^cd	0.321^d	0.279^d	0.313^d	0.350^cd	0.318^d	0.080	<0.0001	0.0223	0.0039
NF-κBp50	1.021^ab	1.215^a	0.912^ab	0.818^ab	0.766^ab	0.754^ab	0.582^b	0.818^ab	0.652^ab	0.749^ab	0.138	0.2871	0.1205	0.2039
NF-κBp65	1.021^abc	1.143^a	0.965^abc	0.875^bc	0.988^abc	0.986^abc	0.798^c	1.074^ab	1.109^ab	0.934^abc	0.078	0.1036	0.7018	0.9137
Nrf2	1.038^de	0.592^e	1.305^cd	1.500^cd	1.697^bc	2.106^ab	2.531^a	2.347^a	2.096^ab	1.488^cd	0.123	<0.0001	0.0743	<0.0001

GPx1 = glutathione peroxidase 1; GPx4 = glutathione peroxidase 4; NF-κBp50 = nuclear factor kappa-Bp50; NF-κBp65= nuclear factor kappa-Bp65; Nrf2 = nuclear factor erythroid 2-related factor. The cells were randomly divided into 10 groups with 6 replicates, and 3 independent experiments were performed. ^a–fMeans in the same row not followed by the same lowercase superscript letters differ significantly (P < 0.05), whereas the differences were considered to be a statistical trend when 0.05 < P < 0.10. The number of observations for each mean value was 6 (n = 6).