Heat load altered protein kinase C signaling. Following 12 h of HS, PKC signaling: Phosphorylated Protein Kinase C (pPKCδ/θ Ser643/676), Phosphorylated PKCζ/λThr410/403 protein expressions were measured by Western blot in the TN, HS and PFTN groups in whole homogenate and (A, B) in membrane fraction (C, D). Representative blots are included. Ponceau S stain (PS) was used as a loading control. Values are mean ± SE; n = 8/group. *Indicates significantly different from TN (P < 0.05); #indicates significantly different from HS (P < 0.05).