Heat load altered glycogen metabolism signaling. Following 12 h of HS Glycogen synthase kinase 3 beta (GSK3β), Phosphorylated GSK3β (pGSK3βSer9), Phosphorylated GSK3β (pGSK3βThy216), Glycogen synthase (GS), and Phosphorylated GS (pGSSer641) protein expressions in whole homogenate were measured by Western blot in the TN, HS, and PFTN groups (A, B). Representative blots are included. Ponceau S stain (PS) was used as a loading control. Values are mean ± SE; n = 8/group. *Indicates significantly different from TN (P < 0.05); #indicates significantly different from HS (P < 0.05).