Table 2.
PEDV detection in feces of pig inoculation with PEDV at dpi 2, 5, 10, 15, and 201
| Parameter | Dpi | SEM | P-value | ||||
|---|---|---|---|---|---|---|---|
| 2 | 5 | 10 | 15 | 20 | |||
| Ct2 | 29.15a | 21.01b | 31.52a | 32.69a | 33.50a | 1.826 | <0.001 |
| Positive/Total | |||||||
| PCR3 | 5/8 | 8/8 | 6/8 | 2/8 | 1/8 | – | – |
| ISH4 | – | 7/8 | – | – | 0/8 | – | – |
| IHC5 | – | 7/8 | – | – | 0/8 | – | – |
1Control pigs remained negative for PEDV throughout the duration of the study.
2Ct, cycle threshold. Ct value ≥35 is considered negative for PEDV.
3Positive pigs out of total pigs swabbed at that dpi. Pigs were considered positive for PEDV presence in feces when Ct <35.
4Positive pigs were those that PEDV nucleic acid was detected in the jejunum.
5Positive pigs were those that PEDV protein was detected in the jejunum.
a,bMeans without a common superscript differ (P ≤ 0.05).