Table 6.

Proteolysis markers in LD of naïve (Control) and PEDV-inoculated pigs at dpi 5 and 20¹

Parameter	Dpi				SEM	P-value
	5		20
	Control	PEDV	Control	PEDV		trt	dpi	trt × dpi
Calpastatin, AU2	1.00	1.05	0.40	0.77	0.125	0.092	<0.001	0.201
µ-Calpain,3 %
80 kDa	44.28	47.54	54.18	52.84	3.802	0.802	0.051	0.548
78 kDa	18.74	16.03	26.42	28.03	3.259	0.865	0.004	0.511
76 kDa	36.98	36.44	19.40	19.14	3.408	0.907	<0.001	0.967
Myofibrillar protein,4 mg	31.78	32.89	23.68	24.35	2.250	0.696	0.009	0.921
ERMs, mg	0.06	0.06	0.05	0.07	0.01	0.303	0.698	0.247
ERMs,5 %	0.20	0.20	0.22	0.31	0.041	0.324	0.112	0.325
Protein:RNA	24.69	26.31	25.20	23.29	2.807	0.958	0.659	0.534
20S proteasome6	20.97b	28.59a	17.24b	9.81c	2.477	0.970	<0.001	0.005

trt, treatment.

¹Estimates are represented by eight observations per treatment per time.

²AU = arbitrary units relative to Control at dpi 5.

³Protein density percentages of unautolyzed 80 kilodalton µ-calpain subunit and its 78 and 76 kilodalton subunit autolysis products.

⁴Obtained from 1.5 g frozen LD.

⁵Represented as a percent of crude myofibrillar protein extraction.

⁶20S proteasome activity measured as micromolar (µM) of released fluorescent 7-amino-4-methylcoumarin (AMC) from LLVY-AMC per mg protein.

^a,bMeans without a common superscript differ (P ≤ 0.05).