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. 2018 Feb 8;96(1):17–26. doi: 10.1093/jas/skx024

Figure 2.

Figure 2.

Effect of Six1 promoter (AC)n microsatellite variations on promoter activity. Promoter activity analyses were performed with Dual-Luciferase Reporter Assay system (Promega) in C2C12 and PK15 in vitro. PGL3-Basic-(AC)19 and PGL3-Basic-(AC)22 represent the promoters derived from (AC)19/19 and (AC)22/22 homozygous genotype pigs, respectively. Each group was performed in six repetitions (n = 6) and data were presented as mean ± SE. Statistical analysis and graph drawing were conducted using GraphPad Prism (5.0), and an unpaired Student’s t-test was used to evaluate the statistical significance between the two groups. ***The significant effect at P < 0.001.