Table 1.
Primers used in this study
| Gene name | Length of fragment | Primers* | Location | Application |
|---|---|---|---|---|
| Six1 | 247 | F1: CTCCCACTCCCACTTATGTCTCA R1: ATGCAAGGAGATAAGGGGAGCCA |
Chr1:189625957-189626204 | Amplification |
| Six1 | 307 | F2: TCAGAGYTAACTAGAGCCATAAAGTCACT R2: TGTGTAGATTGCAATGCTGGCCT |
Chr1:189625716-189626023 | Amplification |
| Six1 | 255 | F3: GGACATCTCTCATTCTCTGGCAGT R3: CCCTGGGAACTTCACACGAAAGG |
Chr1:189625517-189625768 | Amplification |
| Six1 | 326 | F4: CCTCTCAGAACAGGGCAAGGATT R4: AAAGGCCGCTACTATTTGGGACA |
Chr1:189625301-189625627 | Amplification |
| Six1 | 313 | F5: TCAGATGTGGAAACTGAGCCCA R5: ACGCGATAAATAATACAAGAGCAAGCA |
Chr1:189625131-189625444 | Amplification |
| Six1 | 304 | F6: GGCAGGAGGGAAGAGAGGAGAAA R6: CCTCTGCAGTGTCATCTAAGGGC |
Chr1:189624938-189625242 | Amplification |
| Six1 | 316 | F7: AGTCATTGATTTGTGCAGAGATGAGAGCGGCT R7: AGTGTCCCCGGCGCGCTGATTGG |
Chr1:189624677-189624993 | Amplification |
| Six1 | 305 | F8: TCGCGGTCCCACTCCTCCCACCA R8: TGAGTAGGGCGCGCACTGGACAGG |
Chr1:189624532-189624837 | Amplification |
| Six1 | 305 | F9: AACGAGCAGCATCCACCCGGCGG R9: TGTGTGAAGCCGAACGATGGCAGCA |
Chr1:189624324-189624629 | Amplification |
| Six1 | 236 | F10: CTCTGGACACCACTTCCCATGAC R10: ACAAGCACGCATTTAGAAGTCACT |
Chr1:189622076-189622312 | Amplification |
| Six1 | 329 | F11: CCGTCCGTCCTTTAAGTCAG R11: AGCACGCATTTAGAAGTCAC |
Chr1:189622079-189622408 | PCR–RFLP genotyping |
| Six1 | PF: cggGGTACCATTGTTCGGTGGTGCTGAG PR: cggCTCGAGAAAGGCCGCTACTATTTGG |
Chr1:189625301-189626173 | Promoter activity analysis | |
| Six1 | 189 | F: CGTGTTGCGGGAGTGGTA R: TGCTTGTTGGAGGAGGAGTT |
Chr1:189621136-189623371 | Expression analysis |
| HPRT | 134 | F: GGTCAAGCAGCATAATCCAAAG R: CAAGGGCATAGCCTACCACAA |
ChrX:110352282-110356641 | Expression analysis |
*Restrictive enzyme sites are marked by underline and bold type, and protective base are indicated by bold lowercase.