Figure 2.
Demonstration of codon 178 mutation by allele-specific oligonucleotide hybridization. Duplicate filters were hybridized with oligonucleotides that recognize either the wild type allele, Asp 178 on the left, or the mutant allele, Asn 178 on the right. (A-C) The two columns show hybridization of the oligonucleotides to DNA amplified from two affected members with the Asn 178 mutation from the first three kindreds in table 1.(D) An unaffected member from the Berg kindred (IV-2, figure 1).