Table 3. Expected sizes of DNA fragments for the three possible genotypes of each codon of the beta-tubulin gene of A. lumbricoides and N. americanus after digestion with the appropriate restriction enzymes.
Codon | Genotype | Fragments (bp) | |
---|---|---|---|
A. lumbricoides | 167 | Mutated homozygous (TAC/TAC) | 404, 139, 65 |
Unmutated homozygous (TTC/TTC) | 543, 65 | ||
Heterozygous (TTC/TAC) | 543, 404, 139, 65 | ||
198 | Mutated homozygous (GCA/GCA) | 500, 108 | |
Unmutated homozygous (GAA/GAA) | 608 | ||
Heterozygous (GAA/GCA) | 608, 500, 108 | ||
N. americanus | 198 | Mutated homozygous (GCG/GCG) | 315 |
Unmutated homozygous (GAG/GAG) | 262, 53 | ||
Heterozygous (GAG/GCG) | 315, 262, 53 | ||
200 | Mutated homozygous (TAC/TAC) | 315 | |
Unmutated homozygous (TTC/TTC) | 242, 73 | ||
Heterozygous (TTC/TAC) | 315, 242, 73 |