Table 1. In vitro substrate formulation (mg/g DM) and chemical composition.
| Substrates1 | |||||
|---|---|---|---|---|---|
| Whole forage | SN | ISN | NDIN | ||
| Forage | 1000 | 0 | 0 | 0 | |
| SN | 0 | 330–5622 | 0 | 0 | |
| ISN | 0 | 0 | 670–684 | 0 | |
| NDIN | 0 | 0 | 0 | 507–536 | |
| Soybean meal | 0 | 0–198 | 109–212 | 163–262 | |
| NDF | 0 | 438–480 | 0 | 0 | |
| CHO3 | 0 | 0 | 111–205 | 196–300 | |
| Chemical composition | |||||
| DM, g/kg | 860–940 | 936–950 | 940–950 | 940–950 | |
| CP, g/kg DM | 132–166 | 142–168 | 147–159 | 143–155 | |
| NDF, g/kg DM | 500–550 | 480–546 | 500–547 | 500–547 | |
1 Substrates containing: SN–soluble N fraction; ISN–insoluble N fraction; NDIN–neutral detergent-insoluble N fraction.
2 The substrates were prepared to have similar CP and NDF concentrations. Since concentrations of CP and/or NDF in the same fraction differed between forages, the ratios of substrate components varied.
3 CHO–carbohydrate mixture containing pectin, starch and maltose (2:1:1 on DM basis).