Table 1.
Gene | Forward primer | Reverse primer |
---|---|---|
GAPDH | 5′-AGGGCTGCTTTTAACTCTGGT-3 | 5′-CCCCACTTGATTTTGGAGGGA |
IL-6 | 5′-ACAACCACGGCCTTCCCTACTT | 5′-CACGATTTCCCAGAGAACATGTG |
MCP-1 | 5′-CCACTCACCTGCTGCTACTCAT | 5′-TGGTGATCCTCTTGTAGCTCTCC |
TNF-α | 5′-CAGCCTCTTCTCCTTCCTGAT | 5′-GCCAGAGGGCTGATTAGAGA |
IL-1β | 5′-AACCTGCTGGTGTGTGACGTTC | 5′-CAGCACGAGGCTTTTTTGTTGT |
Abbreviations: IL, interleukin; qPCR, quantitative polymerase chain reaction; TNF, tumor necrosis factor.