Figure 1.

Physical stability of SN-38 liposome preparations.

Notes: (A) Optical density measurements of SN-38 liposome suspensions. The measurements were conducted for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples immediately after preparation, and also 24, 72, and 168 h after preparation, from dark blue to light blue. Samples were stored at 4°C in the dark. (B) Size and (C) SN-38 retention rate during storage of lyophilized SN-38 loaded liposomes. The measurements were undertaken for each formulation indicated in the x-axis. The grup of bars for each formulation corresponds to samples after rehydration of freshly lyophilized samples and at 24 and 168 h after storage, from dark pink to light pink. The measurements were performed, from left to right, after rehydration of freshly lyophilized samples and 24, and 168 h after storage. Data correspond to mean values ± SD of, at least, three different experiments.

Abbreviations: SLE, soy bean lipid extract; SN-38, irinotecan metabolite; DOC, DSPC/DOPS/CHOL; DSPC, L-α-distearoyl-phospathidylcholine; DOPS, L-α-dioleoyl-phospathidylserine; CHOL, cholesterol; EPC, egg yolk phosphatidylcholine; Zave, Z-average mean.