Figure 5.

Uptake of SN-38 by HeLa and Caco-2 cells.

Notes: The internalization of the drug was quantified by flow cytometry. Emission fluorescence at 450 nm was determined after excitation at 405 nm. Data are the mean ± SD from three independent experiments. Significance was assessed using a Student’s paired t-test; P-values <0.02 (*), <0.06 (**), and <0.003 (***). Fluorescence profiles are representative of the three experiments analyzed. The color of the curves indicate the different incubation times: 0 h (black); 3 h (turquoise blue); 6 h (blue); 24 h (red) and 48 h (lilac).

Abbreviations: SN-38sol, irinotecan metabolite solution in DMSO; SN-38lip, irinotecan metabolite in liposomal form.