Figure 5.

ET-1 promotes cell migration and EMT expression by downregulating miR-300 expression in OSCC cells.

Notes: (A) Cells were incubated with ET-1 (10–100 nM) for 24 hours, and miR-300 expression was examined by q-PCR. Cells were transfected with a control miRNA or miR-300 mimic for 24 hours and then stimulated with ET-1 (100 nM) for 24 hours. (B) Invasion and (C, D) EMT marker expression were examined by Transwell invasion assay, RT-qPCR and Western blot. *P<0.05.

Abbreviations: EMT, epithelial–mesenchymal transition; ET-1, endothelin-1; OSCC, oral squamous cell carcinoma; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.