Skip to main content
. 2018 Aug 23;3(16):e120794. doi: 10.1172/jci.insight.120794

Figure 2. Aloxe3 and trehalose induce the PPARγ ligand 12-KETE in murine hepatocytes.

Figure 2

(A) Arachidonic acid metabolism mediated by lipoxygenases 12-LOX, 5-LOX, and eLOX3 (adapted from refs. 26, 29, 58, 59). (B) Lipoxygenase activity in primary murine hepatocytes upon overexpression of β galactosidase or Aloxe3. n = 3 from 1 representative experiment of 2 experiments with similar results. (C and D) Quantitative GC-MS analysis of the stable lipoxygenase reaction products 12-KETE, 5-HETE, and 12-HETE. Data represent 4 independent cultures per group from 1 experiment, representing 2 independent experiments with similar results. **P < 0.01 and ***P < 0.001 by 2-tailed t test with Bonferroni-Dunn post hoc correction versus untreated controls group or versus the bracketed comparison group where indicated.