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. 2018 Aug 23;3(16):e122156. doi: 10.1172/jci.insight.122156

Figure 2. Biochemical and metabolic adaptive responses to fasting are intact in 4-month-old eNOS–/– mice.

Figure 2

(A) Ex vivo quantification of palmitate oxidation rate in the livers of wild-type, eNOS–/–, eNOS S1176A (eNOS SA), and eNOS S1176D (eNOS SD) mice (n = 4–8) after 20 hours of food withdrawal expressed as fold change from the rate quantified at fed state. (B) Respiratory exchange ratio (RER). Circles, wild-type mice; squares, eNOS–/– mice (n = 8). (C) Depletion and restoration of liver glycogen levels in response to fasting. (D) Circulating levels of β-HB. The white boxes correspond to wild-type mice and the gray boxes correspond to eNOS–/– mice (n = 8). (E) Hepatic levels of succinyl-CoA and (F) the ratios of succinyl-CoA to acetyl-CoA and propionyl-CoA to acetyl-CoA. Scatter dot plots indicate mean ± SD. Box-and-whisker plots show median, 25th and 75th percentiles, and minimum and maximum values. Data were analyzed by 2-way ANOVA (A–D), and by 1-way ANOVA (E and F) *P < 0.05, ***P < 0.001.