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. 2018 Aug 23;3(16):e99791. doi: 10.1172/jci.insight.99791

Figure 5. Tumor-derived IL-6 and CXCL8 drive osteosarcoma (OS) lung colonization.

Figure 5

(A) Flow cytometry for expression of surface receptors in cells modified with lentiviral constructs to inducibly express shRNAs targeting IL-6ST or both CXCR1 and -2 (single construct). (B) ELISA analysis of IL-6 and CXCL8 performed on 72-hour supernatants from OS-17 cell cultures bearing similar lentiviral constructs targeting cytokine production. (C) Quantification of metastatic colonies from lungs removed from mice inoculated with OS-17 cells bearing the inducible shRNA constructs. Mice were either maintained on water supplemented with doxycycline or with standard water. At the time that one mouse in either group met endpoint criteria, all mice within that study were euthanized and lungs harvested and metastases (Mets) counted. Scale bar: 2 mm. Representative lung sections shown (n = 5 or 10 mice per group). *P < 0.05 by Mann-Whitney U test.