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. 2018 Aug 23;3(16):e99791. doi: 10.1172/jci.insight.99791

Figure 6. Tumor-derived IL-6 and CXCL8 driven by aberrant ΔNp63 expression is critical for osteosarcoma (OS) lung colonization.

Figure 6

(A) Knockdown of ΔNp63 using an inducible shRNA construct demonstrates strong reduction in p63 expression. (B) This reduction correlates with a loss of both IL-6 and CXCL8, while lentivirus-transduced cells overexpressing ΔNp63 increase production of IL-6 and CXCL8. (C) Mice inoculated with inducible-knockdown ΔNp63 constructs demonstrate decreased lung colonization when maintained on IPTG-containing water. Scale bar: 2 mm. (D) Mice inoculated with OS-25 cells transduced with a vector expressing ΔNp63 show a gain of lung colonizing capacity relative to empty vector–transduced cells. When those same cells are also transduced with shRNAs targeting both IL-6 and CXCL8, the increased lung colonization capacity is lost. Scale bar: 500 μm. Representative lung sections shown (n = 5 or 10 mice per group). *P < 0.05; **P < 0.01 by Mann-Whitney U test. Mets, metastases.