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. 2018 Aug 23;3(16):e99791. doi: 10.1172/jci.insight.99791

Figure 7. Interactions of OS-17 cells with bronchial epithelial and smooth muscle cells stimulate IL-6 and CXCL8 production and migration/invasion.

Figure 7

(A) Supernatants from cell cultures of serum-starved OS-17 grown alone or together with human bronchial smooth muscle cells (SMCs) or human bronchial epithelial cells (HBECs) for 48 hours were analyzed by ELISA for IL-6 or CXCL8. Coculture stimulated production of both IL-6 and CXCL8. (B) Supernatants from serum-starved OS-17 cultured for 48 hours in fresh mixed media or in media containing cell-free supernatants from HBECs or SMCs were likewise evaluated using ELISA. Both sets of conditioned supernatants stimulated IL-6 and CXCL8 production far above baseline. (C) Transwell migration using FBS or conditioned supernatants (supes) from HBECs or BSMCs as the chemoattractant. (D) Further refinement of tumor–primary cell interactions that drive production of IL-6 and CXCL8. After serum starvation, osteosarcoma cells were exposed to the respective media alone or to this plus the primary cells in the presence or absence of inhibitors of IL-6 and CXCL8 signaling. P values shown relative to coculture condition (A), to fresh media (B and C), or to coculture plus vehicle (D). **P < 0.01; ***P < 0.001; ****P < 0.0001 by 1-way ANOVA with Tukey’s post hoc test.