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. 2018 Aug 23;3(16):e99543. doi: 10.1172/jci.insight.99543

Figure 3. Repetitive bleomycin treatment results in cellular hypoxia localized to type II AECs.

Figure 3

(A) Pimonidazole injection (i.p., 60 μg/g) was performed 3 hours before euthanasia in mice treated with repetitive intratracheal bleomycin, followed by immunostaining for pimonidazole adducts on lung sections. Scale bars: 800 μm (low magnification): 80 μm (high magnification). (B) Dual immunofluorescence for pimonidazole adducts (red) and pro-SPC for type II AECs (green). Nuclei were counterstained with DAPI (blue). White arrows point to cells with colocalization (yellow). Scale bar: 80 μm. (C) Quantification of the percentage of pro-SPC+ cells that were also positive for pimonidazole per high-power field (HPF).