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. 2018 Aug 23;3(16):e99543. doi: 10.1172/jci.insight.99543

Figure 7. CHOP mediates hypoxia-induced apoptosis of AECs through expression of apoptosis-regulating proteins.

Figure 7

MLE12 cells were transfected with CHOP siRNA or nontarget (NT) siRNA and exposed to hypoxia for 48 hours. (A) Western blot for CHOP. β-Actin was used as a loading control. (B) Cell death as measured by LDH assay. Comparisons between groups were made using 1-way ANOVA with Tukey’s post hoc test. *P < 0.05 compared with NT siRNA + 1.5% O2, **P < 0.05 compared with NT siRNA + 21% O2. (C) Volcano plot showing differentially regulated genes (cycle threshold < 30) using the Mouse Apoptosis RT2 Profiler PCR Array comparing MLE12 cells treated with CHOP siRNA or NT siRNA, followed by hypoxia exposure for 48 hours. n = 3 samples in each group.