Skip to main content
. Author manuscript; available in PMC: 2018 Sep 17.
Published in final edited form as: Nature. 2009 Sep 24;461(7263):515–519. doi: 10.1038/nature08249

Fig. 3.

Fig. 3

Restrictive substitutions impede evolutionary reversibility. a) When group W substitutions are restored to their ancestral state (w), the nonfunctional AncGR2-xyz is rescued, and the ancestral sensitivity to all three ligands is restored. Fold increase in luciferase reporter expression is shown with cortisol (purple), aldosterone (solid blue), and DOC (dashed blue). b) Group W substitutions all impede reversiblility: restoring the ancestral states singly (Y107A) or in structurally interacting pairs (Q84H/C91Y and Q114G/M197L) partially rescues AncGR2-xyz.