Figure 4. Parameters describing orientation-tuned neurons preferring the rewarded or non-rewarded stimulus location.

(a) Left panel: Mean ± SEM percentage of significantly orientation-tuned cells per mouse. ‘A’: All locations. ‘R’: Rewarded location. ‘N’: Non-rewarded location. (b) Absolute fraction of orientation-tuned neurons responding to the rewarded (red) and non-rewarded (blue) location separately, and relative to the trained orientation (e.g. neurons with a preferred orientation nearly identical to the trained orientation were in bin 0°−10°). Bars above data points indicate relative bin-widths. Note that because the left-most bin (0–10 degrees) is only half the width of the other bins, it is expected to contain half of the fraction of the other bins. (c) Distribution (normalized per mouse and bin-width) of preferred orientations relative to the trained orientation (given a flat distribution of preferred orientations, the expected fraction of each bin would be 1.0). (d) Difference in fraction of orientation tuned neurons to the rewarded minus the non-rewarded location (positive values indicate a larger fraction of neurons tuned to the rewarded stimulus location; *p=0.042, Kruskal-Wallis test, post hoc WMPSR test). (e) The mean (±SEM) ∆F/F response to the preferred direction as a function of the preferred orientation relative to the trained orientation for rewarded and non-rewarded location-tuned neurons separately (*p<0.05, WMPSR test). The lines that fall between 0% to 0.5% ∆F/F show the response amplitude of the same neurons, but to the orientation that was orthogonal to the preferred orientation. We note that the sequence of bins is thus not representative of the entire tuning curve but shows the response amplitude to a specific (preferred or orthogonal) direction. (f) Same as e), but for bandwidth (*p<0.05, WMPSR test). (g) As in e), but for sparseness of the tuning curve response calculated across the population all neurons. For all panels: Red colors indicate groups of cells preferring ‘rewarded’ conditioned orientations, while blue colors indicate groups of cells preferring ‘non-rewarded’ trained orientations. Both colors fade to gray, indicating that the preferred orientation of the cells becomes more dissimilar from the trained orientation.

Figure 4—figure supplement 1. Comparison of orientation tuning parameters between cells located close and far away from the rewarded/non-rewarded border.

(a) Distribution of preferred orientation of orientation-tuned neurons relative to the trained orientation (mean per bin, averaged ± SEM across mice). 'A': All locations. 'F': Full imaging regions. 'B': Border imaging regions. (b) The mean (±SEM, averaged across mice) ∆F/F response to the preferred direction as a function of the preferred orientation relative to the trained orientation. (c) Idem, but for the tuning curve bandwidth. For all panels, solid lines: Data from the Full rewarded and Full non-rewarded regions of the visual cortex. Dashed lines: Data from the Border rewarded and Border non-rewarded regions of the visual cortex. All performed tests compared data from Full and Border regions across mice, no significant differences were observed.