FIG 5.

Analysis of apoptosis and proliferation of maternally engrafted and autologous peripheral T cells in patient 1 (P1). A, Staining with Annexin V reagent in freshly isolated CD4⁺ and CD8⁺ T cells from patient 1. B, Staining of CD4⁺ and CD8⁺ T cells with Annexin V reagent after 3 days of culture in the indicated stimuli for an adult control subject (red), HLA-A2⁻ autologous cells from patient 1 (blue), and HLA-A2⁺ maternally engrafted cells from patient 1 (green). C, Cells from an adult control subject, patient 1 cultured in bulk, or patient 1 magnetically separated into HLA-A2–expressing and nonexpressing fractions were cultured for 3 days after CFSE labeling. HLA-A2 expression profile after culture (left) and CFSE and HLA-DR expression (right). D, Proliferation of T cells from patient 1 and her mother to irradiated allogeneic targets from the indicated donors in mixed lymphocyte culture.