Table 2.
Statistics of transcriptomes assembled on CLC Genomics Workbench 8.0.1 using the following parameters: kmer size = 21, minimum contig length of 200 and mapping options as default (mismatch cost: 2, insertion cost: 3, deletion cost: 3, length fraction: 0.5, similarity fraction: 0.8).
| Parameters | Untreated Control Adult Females | Untreated Control Adult Males | Permethrin-Treated Surviving Adult Males | Permethrin + PBO-Treated Killed Adult Males |
|---|---|---|---|---|
| Number of raw reads | 134,671,818 | 132,374,494 | 68,856,572 | 65,427,160 |
| Number of raw reads post trimming | 126,531,116 | 127,276,458 | 66,325,598 | 61,732,240 |
| Number of contigs (>200 nt) | 15,699 | 11,961 | 2672 | 7278 |
| Total size of contigs (nt) | 15,836,681 | 10,217,787 | 2,048,521 | 6,733,471 |
| Longest contig (nt) | 20,187 | 21,272 | 16,352 | 33,914 |
| Average contig length (nt) | 1,009 | 854 | 767 | 925 |
| N50 (nt) | 1607 | 1267 | 988 | 1341 |
| Number of contigs > 500 bp (%) | 9331 (59%) | 6452 (54%) | 1264 (47%) | 4094 (56%) |
| Number of contigs > 1000 bp (%) | 5386 (34%) | 3136 (26%) | 477 (18%) | 1922 (26%) |
| Contigs with BlastX hits (%) | 8778 (56%) | 7064 (59%) | 1609 (60%) | 4472 (61%) |
| Contigs with InterProScan (%) | 10,331 (66%) | 8392 (70%) | 1992 (75%) | 5561 (76%) |
| Contigs with GO Mapping (%) | 8770 (56%) | 7056 (59%) | 1609 (60%) | 4463 (61%) |
| Contigs with Enzyme Codes (%) | 2963 (19%) | 2449 (20%) | 641 (24%) | 1628 (22%) |
| Contigs with KEGG Pathway (%) | 2183 (14%) | 1765 (15%) | 495 (19%) | 1211 (17%) |