Subject area	Biology
More specific subject area	Insect transcriptome
Type of data	Transcriptome sequences and associated annotations (tables, text file)
How data was acquired	2×54 paired-end read RNAseq of RNA isolated from whole newly emerged unfed adult flies
Data format	Raw FASTQ and processed FASTA sequence files, including assembled transcriptome FASTA files
Experimental factors	Isolates: Newly emerged unfed adult females, newly emerged unfed adult males, newly emerged unfed adult males treated with permethrin, newly emerged unfed adult males treated with permethrin + piperonyl butoxide
Experimental features	Assembled transcriptomes of whole body of newly emerged unfed adult flies (Untreated Control Adult Females, Untreated Control Adult Males, Permethrin-Treated Surviving Adult Males and Permethrin + Piperonyl Butoxide-Treated Killed Adult Males)
Data source location	St. Gabriel, Louisiana, USA
Data accessibility	Data is with this article and also available at the National Center for Biotechnology Information (NCBI) Short Read Archive (SRA) through the direct link https://www.ncbi.nlm.nih.gov/sra/SRP131897 or through SRA accession number SRP131897. The adult horn fly transcriptome Shotgun Assembly project has been deposited at DDBJ/EMBL/GenBank under the accession GGLM00000000. The version described in this paper is the first version, GGLM01000000. The overall BioProject ID is PRJNA429442 and the BioSample accessions are SAMN08355023, SAMN08355024, SAMN08355025, and SAMN08355026.