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. 2018 Sep 17;8:13939. doi: 10.1038/s41598-018-32396-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) PCR amplification of the nptII gene produced 786 bp of the amplicon. M- Marker (1 kb plus ladder), PC-Positive Control, NC-Negative Control. P4, P7, P8 & P12 are the putative transformants. (Full-length gel image presented in Supplementary Fig. S1). (b) PCR amplification of rol B gene produced 400 bp of the amplicon. M-marker (1 kb plus ladder), PC -Positive Control, NC -Negative Control, P4, P7, P8 & P12 are the putative transformants. (Full-length gel image presented in Supplementary Fig. S2). (c) Evaluation of putative transgenic hairy root lines by Southern hybridization. M-marker (1 Kb ladder), PC-Positive Control, NC- Negative Control, P4, P7, P8, P11, and P12 are putative transgenic hairy root lines. (Full-length gel image of the southern blot with the visible molecular marker have presented in Supplementary Fig. S3).