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. 2018 Sep 17;9(10):938. doi: 10.1038/s41419-018-0970-6

Fig. 7. Analyses of bone histomorphomertry and osteogenic activity of bone marrow mesenchymal cells.

Fig. 7

HSP60Tg mice showed minor responses to the glucocorticoid-induced loss of (a) BV/TV (scale bar, 120 μm), b Atg4, and Atg12 expressions, (c) RPTOR, and LC3-II levels, and LC3-II/LC3-I ratio. HSP60 overexpression (d) reduced levels of Bax and cleaved caspase3 but (e) increased LC3 immunostaining in osteoblasts (arrows) in glucocorticoid-treated bone tissue. Scale bar, 8 μm. HSP60 overexpression mitigated the glucocorticoid-induced loss of f Runx2, collagen 1α1, and osteocalcin expressions, and g mineralized nodule formation of bone marrow mesenchymal cells. Scale bar, 46 μm. Experiments were performed on six mice, and results are expressed as mean ± standard error. Asterisks (*) resemble a significant difference (P < 0.05) vs. wild-type group, and hashtag (#) indicates a distinguishable difference (P < 0.05) between the group of interest vs. glucocorticoid-treated wild-type group