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. 2018 Sep 17;8:13904. doi: 10.1038/s41598-018-32272-x

Figure 5.

Figure 5

EEF1α1 protein expression levels parallel cellular stress levels. (A) Western blots showing eEF1α1 and β-actin protein levels in MCF10A and MDA-MB-231 breast cancer cell lines. (B) Quantification of β-actin-normalized eEF1α1 protein levels in MCF10A and MDA-MB-231 cell lines using the Western blots shown in (A). (C) Bar graph of normalized eEF1α1 protein levels in MCF10A and MDA-MB-231 cell lines using mass spectrometric quantification (each n = 2)42. (D) Protein expression levels of the heat shock response-inducible HSP90 isoforms α1 and α2. (E) Model explaining the contradictory EEF1A1 mRNA and eEF1α1 protein misexpression in breast carcinoma.