Table 1.
Patient related information and IC50 values in the primary cells
| ID | Age | Stage and histology | Treatment status | Source for primary culture | IHC | IHC | IC50 (µM) | ||
|---|---|---|---|---|---|---|---|---|---|
| Post op 6 cycles chemo (C + P) | Progression at 6 months | Ascites | Chemo naive | PAX8 | WT1 | ||||
| PCAST-1 | 65 | IIIC HGCS | √ | no | √ | √ | √ | √ | 34.7 ± 0.02 |
| PCAST-3 | 45 | IVC HGSC | √ | yes | √ | √ | √ | √ | 19.6 ± 0.5 |
| PCAST-4 | 52 | IIIC HGSC | √ | no | √ | √ | √ | √ | 28.5 ± 0.08 |
| PCAST-6 | 52 | IIIB (Clear cell) | √ | no | √ | √ | √ | 23.2 ± 0.02 | |
| PCAST-12 | 40 | IIIC (HGSC) | √ | no | √ | √ | √ | √ | 23.2 ± 0.01 |
| PCAST-14 | 46 | IIIC (HGSC) | x | yes | √ | √ | √ | √ | 22.3 ± 0.02 |
| PCAST-15 | 56 | IIIC (HGSC) | √ | no | √ | √ | √ | √ | 30 ± 0.5 |
| PCAST-16 | 56 | IVB (HGSC) | √ | no | √ | √ | √ | √ | 27.4 ± 0.15 |
| PCAST-17 | 64 | IIIC (HGSC) | x | no | √ | √ | √ | √ | 14.5 ± 0.15 |
Clinical data of patient sample/primary cells includes patient age, stage/histology treatment status and origin. We confirmed the epithelial ovarian cancer cells (EOC) by IHC of various markers (PAX8, WT1). PCAST- primary culture ascites; HGSC- high-grade serous cancer; C + P- carboplatin + paclitaxel.
PCAST primary culture ascites, HGSC high-grade serous cancer, C+P carboplatin + paclitaxel