Table 2.
List of primers used in RT-PCR
| Target Gene | sequence (5′ to 3′) | Annealing temperature (°C) | product size (bp) |
|---|---|---|---|
| Bax | GGGGACGAACTGGACAGTAA | 50 | 490 |
| CCTCCCAGAAAAATGCCATA | |||
| Bcl2 | GGATGCCTTTGTGGAACTGT | 50 | 479 |
| GGTGCTTGGCAATTAGTGGT | |||
| Caspase 3 | TGGAATTGATGCGTGATGTT | 84 | 460 |
| TCAAGCTTGTCGGCATACTG | |||
| Caspase 9 | GCTTAGGGTCGCTAATGCTG | 25 | 490 |
| GTGCTGAACATCCCACAATG | |||
| GAPDH | GTCAGGTCCACCACTGACAC | 55 | 225 |
| GGAAGGACTCATGACCACAG | |||
| LC3B | GGTGAGAAGCAGCTTCCTGT | 50 | 235 |
| TCTCCTGGGAGGCATAGACC | |||
| Beclin1 | GGACACTCAGCTCAACGTCA | 45 | 208 |
| AGCCTGGACCTTCTCGAGAT | |||
| Atg3 | GAGCAACGGCAGCCTTTAAC | 25 | 192 |
| TCCAAGTTCTCCCCCTCCTT | |||
| Atg5 | ACAGATGACAAAGATGTGCT | 50 | 228 |
| TGGTGTGCCTTCATATTCAA | |||
| Atg7 | TGCTATCCTGCCCTCTGTCT | 52 | 193 |
| GCAAGGAAACCAGCACCATG | |||
| Atg12 | AAGTGGGCAGTAGAGCGAAC | 52 | 203 |
| CACGCCTGAGACTTGCAGTA | |||
| E-Cadherin | TCATGAGTGTCCCCCGGTAT | 50 | 240 |
| TCTTGAAGCGATTGCCCCAT | |||
| Snail | GAGGACAGTGGGAAAGGCTC | 52 | 248 |
| TGGCTTCGGATGTGCATCTT | |||
| Slug | CATCTTTGGGGCGAGTGAGT | 52 | 191 |
| GGCCAGCCCAGAAAAAGTTG |