Fig. 6. Bim mediates the antitumor effects of idelalisib in vivo.

a Nude mice were injected s.c. with 5 × 10⁶ WT and Bim-KO HepG2 cells. After 1 week, mice were treated with 30 mg/kg idelalisib or buffer for 10 consecutive days. Tumor volume at indicated time points after treatment was calculated and plotted (n = 6 in each group), **P < 0.01; *P < 0.05 (Student’s t-test). Arrows indicate idelalisib injection. b Representative tumors at the end of the experiment in (a). c WT HepG2 xenograft tumors were treated with 30 mg/kg idelalisib or the control buffer as in (a) for 4 consecutive days. Phosphor-FoxO3a and Bim in representative tumors were analyzed by western blotting and normalized to β-actin. The data represent the mean ± SD of three independent experiments. **P < 0.01; *P < 0.05 (Student’s t-test). d Paraffin-embedded sections of tumor tissues from mice treated as in (a) were analyzed by TUNEL staining. Left, representative TUNEL staining pictures; Right, TUNEL-positive cells were counted and plotted. e Tissue sections from (D) were analyzed by active caspase 3 staining. Left, representative staining pictures; Right, active caspase 3-positive cells were counted and plotted. The results of (d) and (e) were expressed as the means ± SD of 3 independent experiments. **P < 0.01 (Student’s t-test). Scale bars: 25 μm