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. 2018 Sep 17;8:13951. doi: 10.1038/s41598-018-32390-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Toxicity to HeLa cells of cell-free supernatants (a) and hamster lethal activity (b) of isolates HMX-149, HMX-152, and HSJD-312. Isolates HMX-152 and HSJD-312 caused C. sordellii-like CPEs with different potencies and have variant TcdB sequences of the Clade 2 type. By contrast, isolate HMX-149 caused an arborizing CPE and its TcdB sequence clustered with classical TcdBs from Clade 2 strains. Isolate HSJD-312 was more lethal than HMX-149 and HMX-152. A control NAP1 strain was tested in parallel. Pink and blue colors were used to highlight variant and classical TcdBs, respectively.