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. 2018 Sep 17;8:13928. doi: 10.1038/s41598-018-32303-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NNFE on pigmentation of mouse skin exposed to UVB. (A) Fontana-Masson staining of dorsal skin sections from the mice shown in (Fig. 8B) reveals differences in melanin content. (B) After completing UVB treatment, dorsal skin was excised, homogenised, and assayed by western blot using antibodies against tyrosinase, TYRP-1, TYRP-2, and MITF. Equal protein loading was confirmed using anti-β-actin. Arb, arbutin. (C) Quantification and statistical analysis of the band intensities of tyrosinase, TYRP-1, TYRP-2, and MITF obtained by western blot analysis. *p < 0.05, **p < 0.01, versus the non-treated controls, Student’s t-test. (D) Phosphorylation of CREB confirmed by western blot analysis. NNFE, ethyl acetate fraction of N. nouchali flower extract; CA, caffeic acid.