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Infection pattern of IAV Pan/99(H3N2) in 2D cultured A549 cells. A549 cells were seeded in 24 well plates and infected with indicated MOIs of IAV Pan/99(H3N2) for 24 h. Fixed cells were immunohistochemically labelled with anti-nucleoprotein antibody (red channel), and nuclear counter staining was performed using Hoechst stain (blue channel). Stained A549 cells were analysed by fluorescence microscopy. Scale bar: 100 µm.
