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. 2018 Mar 5;15(8):782–793. doi: 10.1038/cmi.2017.163

Figure 1.

Figure 1

The expression levels of IL-4, IL-5, IL-9 and IL-13 in neutrophils are selectively induced by IL-33. (a) Primary neutrophils freshly isolated from bone marrow of C57BL/6 mice were treated with various cytokines and LPS for 24 h. The IL-4, IL-5, IL-9 and IL-13 mRNA expression levels were determined by real-time PCR. (b) The mRNA expression levels of IL-4, IL-5, IL-9 and IL-13 in primary neutrophils treated with different concentrations of IL-33 for 24 h were determined by real-time PCR. (c) Quantitative PCR analysis of IL-4, IL-5, IL-9 and IL-13 mRNA expression levels in neutrophils treated with IL-33 (100 ng/ml) for different times. (d) The protein levels of IL-4, IL-5, IL-9 and IL-13 in the culture medium of primary neutrophils treated with different concentrations of IL-33 for 24 h were determined by ELISA assays. The protein expression of IL-9 in neutrophils treated with 100 ng/ml IL-33 for 24 h were determined by flow cytometry (e) and confocal microscopy (f). Data are shown as mean±s.d. (n=3), representing one of the three independent experiments. *P<0.05, **P<0.01 and ***P<0.001 compared with control group. Data were analyzed by two-way ANOVA analysis using the SPSS software. ANOVA, analysis of variance; ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide.