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. 2018 Sep 17;9:3785. doi: 10.1038/s41467-018-06194-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Crystal structures of 14-3-3β:ExoS and 14-3-3β:ExoT complexes. a Schematic representation of co-expression constructs used for structure determination. Triangles represent the E379A,E381A double mutation. b, d SEC-RALS profiles of the heterotrimeric (b) and heterotetrameric (d) 14-3-3β:ExoS complexes (main peaks). Estimated molecular weights are indicated. c, e Structures of the 14-3-3β:ExoS heterotrimer (c) and heterotetramer (e). The 14-3-3β dimer is shown in gray. Numbers in italics indicate the ExoS residues that were resolved in the electron density. f Detail of the electron density around carba-NAD in the active site of ExoS (2F_obs – F_calc electron density map contoured at 1σ (0.1073 eÅ⁻³)). g Comparison of the ExoS apo and carba-NAD structures. The loop containing F354 is in two different conformations, where the open conformation from the apo structure is marked with an asterisk. Secondary structural elements are numbered. h Structure of the 14-3-3β:ExoT heterotetramer. c, e, g, h ExoS respectively ExoT are shown colored in a gradient from the N-terminus (blue) to the C-terminus (red) of the construct